Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit

• Target:

  NEFA

• Type:

  Colorimetric method

• Field of Research:

  Metabolic Diseases; Glycolysis and Lipid Metabolism

• Assay Principle:

  Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated indirectly by measuring the OD value at 715 nm.

• Assay Performance Time:

  30 min

• Sample Type:

  Animal tissue

• Detection Range:

  0.15-1.5 mmol/L

• Sensitivity:

  0.15 mmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C for 12 months with shading light.

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S0142961222005427

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/S0048969722056844

• Citation 03:

  https://www.sciencedirect.com/science/article/pii/S004896972105405X

• Citation 04:

  https://onlinelibrary.wiley.com/doi/abs/10.1111/pai.13651

• Citation 05:

  https://www.mdpi.com/2072-6643/14/3/440

• Citation 06:

  https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8200454/

• Citation 07:

  https://www.sciencedirect.com/science/article/pii/S0753332220303309

• Citation 08:

  https://www.sciencedirect.com/science/article/pii/S2405844020319769

• Citation 09:

  https://link.springer.com/article/10.1007/s10787-018-0519-4

• Citation 10:

  https://www.jstage.jst.go.jp/article/jos/71/5/71_ess21389/_article/-char/ja/

• Inter CV:

  5.1%

• Intra CV:

  3.3%

• Detection Instrument:

  Microplate reader