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Mouse OC/BGP (Osteocalcin) CLIA Kit

CAT: 0763-E-CL-M0529-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-CL-M0529-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
OC; BGP
UniProt
P86546
Cross Reactivity
This kit recognizes Mouse OC/BGP in samples.No significant cross-reactivity or interference between Mouse OC/BGP and analogues was observed
Target
BGLAP; OT; Bone Gamma-carboxyglutamate Protein; Bone Gla Protein
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Cancer; Signal transduction; Stem cells
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse OC/BGP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse OC/BGP. You can calculate the concentration of Mouse OC/BGP in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
78.13-5000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
46.88 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse OC/BGP concentrations in serum, plasma and other biological fluids.

UniProtKB · P86546

Osteocalcin

OSTCN_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P86546
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Bglap
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
OG1
EC number
—
Processing
Precursor
Secondary accessions
P04641
Protein keywords

Biological process

Biomineralization

Ligand

CalciumMetal-binding

PTM

Cleavage on pair of basic residuesDisulfide bondGamma-carboxyglutamic acid

Molecular function

Hormone

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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