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Mouse OC/BGP (Osteocalcin) ELISA Kit

CAT: 0763-E-EL-M0864-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-M0864-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
OC; BGP
UniProt
P86546
Cross Reactivity
This kit recognizes Mouse OC/BGP in samples. No significant cross-reactivity or interference between Mouse OC/BGP and analogues was observed
Target
BGLAP; OT; Bone Gamma-carboxyglutamate Protein; Bone Gla Protein
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Signal transduction; Stem cells
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse OC/BGP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse OC/BGP. You can calculate the concentration of Mouse OC/BGP in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.39-25 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.23 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202004831
Citation 02
https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202100808
Citation 03
https://www.nature.com/articles/s41467-022-29191-x
Citation 04
https://www.sciencedirect.com/science/article/pii/S1534580722006839
Citation 05
https://www.nature.com/articles/s41413-019-0056-9
Citation 06
https://www.tandfonline.com/doi/abs/10.1080/15548627.2020.1839286
Citation 07
https://www.sciencedirect.com/science/article/pii/S1525001620301842
Citation 08
https://www.nature.com/articles/cdd201672
Citation 09
https://www.sciencedirect.com/science/article/pii/S0891584922005147
Citation 10
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086346/
Citation 11
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019162/
Citation 12
https://www.sciencedirect.com/science/article/pii/S0753332222007703
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse OC/BGP concentrations in serum, plasma and other biological fluids.

UniProtKB · P86546

Osteocalcin

OSTCN_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P86546
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Bglap
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
OG1
EC number
—
Processing
Precursor
Secondary accessions
P04641
Protein keywords

Biological process

Biomineralization

Ligand

CalciumMetal-binding

PTM

Cleavage on pair of basic residuesDisulfide bondGamma-carboxyglutamic acid

Molecular function

Hormone

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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