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Mouse Osteocalcin (OC/BGP) ELISA Kit

CAT: 0013-GTR18854811-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854811-01Size:48 Tests
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Description
Mouse Osteocalcin (OC/BGP) ELISA Kit
Product Name Alternative
BGLAP, BGP, OC, OCN, bone gamma-carboxyglutamate protein, Osteocalcin
UniProt
P86546
Reactivity
Mouse
Target
OC/BGP
Field of Research
Cancer Biology, Infectious Disease & Virology, Musculoskeletal & Connective Tissue Research, Neuroscience, Protein Biochemistry
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
0.23 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse OC/BGP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse OC/BGP. You can calculate the concentration of Mouse OC/BGP in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
0.39-25ng/mL

UniProtKB · P86546

Osteocalcin

OSTCN_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P86546
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Bglap
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
OG1
EC number
—
Processing
Precursor
Secondary accessions
P04641
Protein keywords

Biological process

Biomineralization

Ligand

CalciumMetal-binding

PTM

Cleavage on pair of basic residuesDisulfide bondGamma-carboxyglutamic acid

Molecular function

Hormone

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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