Human AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) CLIA Kit

• Abbreviation:

  AMPK

• UniProt:

  Q13131

• Cross Reactivity:

  This kit recognizes Human AMPK in samples.No significant cross-reactivity or interference between Human AMPK and analogues was observed

• Target:

  AMPK

• Type:

  Sandwich-ELISA

• Applications:

  CLIA

• Field of Research:

  Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction

• Assay Principle:

  This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AMPK. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AMPK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AMPK, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human AMPK. You can calculate the concentration of Human AMPK in the samples by comparing the RLU value of the samples to the standard curve.

• Assay Performance Time:

  3 h 30 min

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  62.5-4000 pg/mL

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Recovery:

  80%-120%

• Sensitivity:

  37.5 pg/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C/-20°C,6 months

• Species:

  Human

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of Human AMPK concentrations in Serum, plasma and other biological fluids.