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Rat C4 (Complement Component 4) ELISA Kit

CAT: 0763-E-EL-R0248-01Size: 24 TestsDry Ice: NoHazardous: No
CAT#:0763-E-EL-R0248-01Size:24 Tests
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Abbreviation
C4
UniProt
P08649
Cross Reactivity
This kit recognizes Rat C4 in samples. No significant cross-reactivity or interference between Rat C4 and analogues was observed
Target
C4
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Immunology
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C4. You can calculate the concentration of Rat C4 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
3.13-200 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
1.88 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat C4 concentrations in serum, plasma and other biological fluids.

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