Human C4 (Complement Component 4) ELISA Kit

CAT: 0763-E-EL-H6027-01Size: 24 TestsDry Ice: NoHazardous: No
CAT#:0763-E-EL-H6027-01Size:24 Tests
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CAS Number
7732-18-5
Abbreviation
C4
Synonyms
C4
UniProt
PN/ACN/AL4, PN/ACN/AL5
Species Reactivity
Human
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Immunology
Detection Method
Colormetric
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C4. You can calculate the concentration of Human C4 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Detection Range
3.13-200 ng/mL
Sensitivity
1.88 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C, 12 months

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