Rat C5a (Complement Component 5a) ELISA Kit

CAT: 0763-E-EL-R3011-01Size: 24 TestsDry Ice: NoHazardous: No
CAT#:0763-E-EL-R3011-01Size:24 Tests
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Abbreviation
C5a
UniProt
P08650
Cross Reactivity
This kit recognizes Rat C5a in samples.No significant cross-reactivity or interference between Rat C5a and analogues was observed
Target
C5a
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cell Biology; Metabolism; Immunology; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C5a. You can calculate the concentration of Rat C5a in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
9.38 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S2001037020304761
Citation 02
https://europepmc.org/article/med/35387346?javascript_support=no
Citation 03
https://link.springer.com/article/10.1007/s12640-019-00026-w
Citation 04
https://journals.lww.com/shockjournal/Fulltext/2017/04000/Tranexamic_Acid_Attenuates_The_Loss_of_Lung.14.aspx
Citation 05
https://www.sciencedirect.com/science/article/pii/S0966327422000338
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat C5a concentrations in serum, plasma and other biological fluids.

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