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Mouse IFNα (Interferon Alpha) ELISA Kit

CAT: 0763-E-EL-M3054-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-M3054-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
IFNα
UniProt
P01572, P01573
Cross Reactivity
This kit recognizes Mouse IFNα in samples.No significant cross-reactivity or interference between Mouse IFNα and analogues was observed
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Immunology; Cancer
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFNα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFNα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFNα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFNα. You can calculate the concentration of Mouse IFNα in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
9.38 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.28203
Citation 02
https://www.sciencedirect.com/science/article/pii/S1742706121005018
Citation 03
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982817/
Citation 04
https://onlinelibrary.wiley.com/doi/abs/10.1111/imm.13379
Citation 05
https://www.sciencedirect.com/science/article/pii/S0223523421008205
Citation 06
https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.13904
Citation 07
https://academic.oup.com/endo/article-abstract/162/12/bqab180/6359040
Citation 08
https://pubs.rsc.org/lv/content/articlehtml/2018/fo/c7fo01161j
Citation 09
https://link.springer.com/article/10.1186/s12865-022-00515-2
Citation 10
https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.14174
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse IFNα concentrations in serum, plasma and other biological fluids.

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