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Human C5a (Complement Component 5a) ELISA Kit

CAT: 0763-E-EL-H0190-03Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-H0190-03Size:96 Tests
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Abbreviation
C5a
UniProt
P01031
Cross Reactivity
This kit recognizes Human C5a in samples. No significant cross-reactivity or interference between Human C5a and analogues was observed
Target
C5a
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cell Biology; Metabolism; Immunology; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C5a. You can calculate the concentration of Human C5a in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
78.13-5000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
46.88 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://europepmc.org/article/pmc/pmc9468265?javascript_support=no
Citation 02
https://academic.oup.com/cei/advance-article-abstract/doi/10.1093/cei/uxac120/6960845
Citation 03
https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0009508
Citation 04
https://www.dovepress.com/by-increasing-the-expression-and-activation-of-stat3-sustained-c5a-sti-peer-reviewed-fulltext-article-CMAR
Citation 05
https://www.physiology.org/doi/abs/10.1152/ajpgi.00464.2013
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human C5a concentrations in serum, plasma and other biological fluids.

UniProtKB · P01031

Complement C5

CO5_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01031
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C5
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q14CJ0, Q27I61
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Biological process

Complement alternate pathwayComplement pathwayCytolysisImmunityInflammatory responseInnate immunity

Disease

Disease variant

Cellular component

MembraneMembrane attack complexSecretedTarget cell membraneTarget membrane

Domain

Signal

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