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Recombinant Rat Fibroblast Growth Factor-basic (rRtbFGF)

CAT: 0013-GTR18991711-01Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18991711-01Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4.
Source
Escherichia coli.
Field of Research
Neuroscience; Stem Cell & Developmental Biology
Endotoxin
Less than 1EU/mg of rRabFGF as determined by LAL method.
Purity
>98% by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells is ≤ 0.2 ng/ml , corresponding to a specific activity of ≥5 x 106 units/mg.
Form
Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.
Molecular Weight
Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 145 amino acids.
Storage Conditions
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
Preservative
Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.
AA Sequence
PALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKYSSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS

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