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RecombinantIL-18, Rat

CAT: 0013-GTR18990522-01Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18990522-01Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Interleukin-18 (IL18, also known as interferon-gamma inducing factor or IGIF) is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. Its biological activity is pleiotropic and it has been shown to induce interferon-gamma production in KG-1 cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells.Recombinant rat Interleukin-18 (rrIL-18) produced in E.coli is a single non-glycosylated polypeptide chain containing 159 amino acids. A fully biologically active molecule, rrIL-18 has a molecular mass of 18.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Product Name Alternative
Interleukin-18, IL18
Source
Escherichia coli.
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE analyses.
Bioactivity
ED50 500 units/mg.
Form
Lyophilized after extensive dialysis against 50mM Tris, 50mM NaCl, pH 8.0.
Molecular Weight
18.4 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant ratInterleukin-18 (rrIL-18) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrIL-18 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Notes
For research use only.
Applications Notes
Reconstituted in ddH2O at 100 μg/ml.
Preservative
Lyophilized after extensive dialysis against 50mM Tris, 50mM NaCl, pH 8.0.
AA Sequence
MHFGRLHCT TAVIRSINDQ VLFVDKRNPP VFEDMPDIDR TANESQTRLI IYMYKDSEVR GLAVTLSVKD GRMSTLSCKN KIISFEEMNP PENIDDIKSD LIFFQKRVPG HNKMEFESSL YEGHFLACQK EDDAFKLVLK RKDENGDKSV MFTLTNLHQS

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