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Flagellin Control protein

CAT: 0013-GTR18957885Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18957885Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Flagellin Control protein
Product Name Alternative
41 kDa antigen, Borrelia burgdorferi p41, fla, Flagellar filament 41 kDa core protein, Bacterial flagellin, control protein
UniProt
P11089
Conjugation
Unconjugated
Source
Borrelia burgdorferi
Origin
Borrelia burgdorferi
Field of Research
Protein Biochemistry
Concentration
1.0 mg/mL
Dilution
ELISA: User Optimized, WB: User Optimized
Purity
Flagellin is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Flagellin and was estimated to be greater than 90% pure.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Flagellin is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 76.3 kDa Flagellin-MBP, (33.9 kDa for Flagellin and 42.4 kDa for MBP) in size corresponding to Flagellin by Western blotting in the appropriate cell lysate or extract. Flagellin Protein was tested in SDS-page and western blot.
Tested Applications
SDS-PAGE, WB
NCBI Accession Number
WP_002661938.1
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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