NPM (Phospho-Thr199) Colorimetric Cell-Based ELISA Kit

• Description:

  The NPM (Phospho-Thr199) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor NPM (Phospho-Thr199) protein expression profile in cells. The kit can be used for measuring the relative amounts of NPM (Phospho-Thr199) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on NPM (Phospho-Thr199) .

• Synonyms:

  B23.1; NPM isoform 1; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin) Numatrin

• Gene Name:

  NPM1

• UniProt:

  P06748

• Reactivity:

  Human, Mouse, Rat

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486) . In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029) .

• Molecular Weight:

  32575 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Nucleophosmin

• Subcellular Location:

  Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.