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MMP-2 Recombinant Protein

CAT: 0013-GTR18911789-01Size: 0.002 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18911789-01Size:0.002 mg
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Description
MMP-2 Recombinant Protein
Product Name Alternative
CLG4, MONA, CLG4A, TBE-1, MMP-II, 72 kDa type IV collagenase, 72 kDa gelatinase, MMP-2
UniProt
P08253
Source
E. coli
Field of Research
Cardiovascular Research; Musculoskeletal & Connective Tissue Research
Purity
Greater than 90% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg) .
Bioactivity
MMP - 2 activity was measured by its ability to cleave a chromogenic peptide MMP - 2 substrate at room temperature. At an MMP - 2 concentration of 2.5 μg/mL, 50% cleavage was achieved at an incubation time of approximately 25 minutes.
Form
Lyophilized
Storage Conditions
The lyophilized MMP-2 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted MMP-2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing MMP-2 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Notes
For research use only.
NCBI Accession Number
NP_004521.1
AA Sequence
MYNFFPRKPK WDKNQITYRI IGYTPDLDPE TVDDAFARAF QVWSDVTPLR FSRIHDGEAD IMINFGRWEH GDGYPFDGKD GLLAHAFAPG TGVGGDSHFD DDELWTLGEG QVVRVKYGNA DGEYCKFPFL FNGKEYNSCT DTGRSDGFLW CSTTYNFEKD GKYGFCPHEA LFTMGGNAEG QPCKFPFRFQ GTSYDSCTTE GRTDGYRWCG TTEDYDRDKK YGFCPETAMS TVGGNSEGAP CVFPFTFLGN KYESCTSAGR SDGKMWCATT ANYDDDRKWG FCPDQGYSLF LVAAHEFGHA MGLEHSQDPG ALMAPIYTYT KNFRLSQDDI KGIQELYGAS PDIDLGTGPT PTLGPVTPEI CKQDIVFDGI AQIRGEIFFF KDRFIWRTVT PRDKPMGPLL VATFWPELPE KIDAVYEAPQ EEKAVFFAGN EYWIYSASTL ERGYPKPLTS LGLPPDVQRV DAAFNWSKNK KTYIFAGDKF WRYNEVKKKM DPGFPKLIAD AWNAIPDNLD AVVDLQGGGH SYFFKGAYYL KLENQSLKSV KFGSIKSDWL GC

UniProtKB · P08253

72 kDa type IV collagenase

MMP2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08253
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MMP2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.24.24
Processing
Precursor
Secondary accessions
B2R6U1, B4DWH3, E9PE45, Q9UCJ8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

AngiogenesisCollagen degradation

PTM

Autocatalytic cleavageDisulfide bondGlycoproteinPhosphoproteinZymogen

Ligand

CalciumMetal-bindingZinc

Cellular component

CytoplasmExtracellular matrixMembraneMitochondrionNucleusSecreted

Disease

Disease variant

Molecular function

HydrolaseMetalloproteaseProtease

Domain

RepeatSignal

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