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Human KRT19 (Keratin 19) Microsample ELISA Kit

CAT: 0013-GTR18873136-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873136-01Size:48 Tests
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Description
Human KRT19 (Keratin 19) Microsample ELISA Kit
Product Name Alternative
K19; CK19; K1CS; Cytokeratin 19; 40-kDa Keratin Intermediate Filament; Keratin, type I cytoskeletal 19
UniProt
P08727
Reactivity
Human
Field of Research
Cancer Biology, Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.133 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT19. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT19. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT19, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT19 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20 ng/mL

UniProtKB · P08727

Keratin, type I cytoskeletal 19

K1C19_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08727
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KRT19
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2R874, Q5XG83, Q6NW33, Q7L5M9, Q96A53, Q96FV1, Q9BYF9, Q9P1Y4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Domain

Coiled coil

Biological process

Host-virus interaction

Cellular component

Intermediate filamentKeratin

PTM

MethylationPhosphoprotein

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