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Human KRT16 (Keratin 16) Microsample ELISA Kit

CAT: 0013-GTR18872114-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872114-01Size:48 Tests
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Description
Human KRT16 (Keratin 16) Microsample ELISA Kit
Product Name Alternative
CK16; NEPPK; Cytokeratin 16; Focal Non-Epidermolytic Palmoplantar Keratoderma; Keratin, type I cytoskeletal 16
UniProt
P08779
Reactivity
Human
Field of Research
Cancer Biology, Infectious Disease & Virology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
0.066 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT16 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P08779

Keratin, type I cytoskeletal 16

K1C16_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08779
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KRT16
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A8K488, P30654, Q16402, Q9UBG8
Protein keywords

Domain

Coiled coil

Technical term

Direct protein sequencingProteomics identificationReference proteome

Disease

Disease variantEctodermal dysplasiaPalmoplantar keratoderma

Cellular component

Intermediate filamentKeratin

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