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Human KRT17 (Keratin 17) Microsample ELISA Kit

CAT: 0013-GTR18872474-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872474-01Size:48 Tests
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Description
Human KRT17 (Keratin 17) Microsample ELISA Kit
Product Name Alternative
PC2; CK17; K17; PC; PCHC1; Cytokeratin 17; Keratin, type I cytoskeletal 17
UniProt
Q04695
Reactivity
Human
Field of Research
Cancer Biology, Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
30 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT17. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT17 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
78.13-5000 pg/mL

UniProtKB · Q04695

Keratin, type I cytoskeletal 17

K1C17_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q04695
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KRT17
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A5Z1M9, A5Z1N0, A5Z1N1, A5Z1N2, A6NDV6, A6NKQ2, Q6IP98, Q8N1P6
Protein keywords

Domain

Coiled coil

Cellular component

CytoplasmIntermediate filamentKeratin

Disease

Disease variantEctodermal dysplasiaPalmoplantar keratoderma

PTM

Isopeptide bondPhosphoproteinUbl conjugation

Technical term

Proteomics identificationReference proteome

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