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Human APOB48 (Apolipoprotein B48) Microsample ELISA Kit

CAT: 0013-GTR18873014-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873014-01Size:48 Tests
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Description
Human APOB48 (Apolipoprotein B48) Microsample ELISA Kit
Product Name Alternative
Apo-B48
UniProt
Q0VD83
Reactivity
Human
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
0.17 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOB48. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOB48. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOB48, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOB48 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.47-30 ng/mL

UniProtKB · Q0VD83

Apolipoprotein B receptor

APOBR_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q0VD83
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
APOBR
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
H3BU97, Q0VD81, Q8NC15, Q9NPJ9
Protein keywords

Coding sequence diversity

Alternative splicing

Disease

Atherosclerosis

Cellular component

Cell membraneChylomicronLDLMembraneVLDL

Biological process

Cholesterol metabolismLipid metabolismLipid transportSteroid metabolismSterol metabolismTransport

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Phosphoprotein

Molecular function

Receptor

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