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Rat APOA4 (Apolipoprotein A4) Microsample ELISA Kit

CAT: 0013-GTR18873352-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873352-01Size:48 Tests
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Description
Rat APOA4 (Apolipoprotein A4) Microsample ELISA Kit
Product Name Alternative
ApoA-IV; ApoAIV; Apo-A4
UniProt
P02651
Reactivity
Rat
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids.
Sensitivity
0.92 μg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APOA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APOA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APOA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APOA4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
3.13-200 μg/mL

UniProtKB · P02651

Apolipoprotein A-IV

APOA4_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P02651
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Apoa4
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
Apo-AIV, ApoA-IV
EC number
—
Processing
Precursor
Secondary accessions
Q5BK92
Protein keywords

Cellular component

ChylomicronHDLSecreted

Biological process

Lipid transportTransport

PTM

Phosphoprotein

Technical term

Reference proteome

Domain

RepeatSignal

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