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Rat TF (Tissue Factor) Microsample ELISA Kit

CAT: 0013-GTR18872908-02Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872908-02Size:96 Tests
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Description
Rat TF (Tissue Factor) Microsample ELISA Kit
Product Name Alternative
CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III
UniProt
P42533
Reactivity
Rat
Field of Research
Cardiovascular Research, Hematology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity
13 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TF in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
31.25-2000 pg/mL

UniProtKB · P42533

Tissue factor

TF_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P42533
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
F3
Protein existence
2: Evidence at transcript level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
TF
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Blood coagulationHemostasis

PTM

Disulfide bondGlycoproteinLipoproteinPalmitate

Cellular component

Membrane

Technical term

Reference proteome

Domain

SignalTransmembraneTransmembrane helix

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