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Rat TF (Tissue Factor) Microsample ELISA Kit

CAT: 0855-ELK2567MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2567MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III
UniProt
P42533
Reactivity
Rat
Field of Research
Hematology; Cardiovascular biology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TF in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Plasma, tissue homogenates, cell lysates and other biological fluids
Detection Range
31.25-2000 pg/mL
Sensitivity
13 pg/mL

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