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Mouse Tissue factor (TF) ELISA Kit

CAT: 0013-GTR18854763-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854763-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse Tissue factor (TF) ELISA Kit
Product Name Alternative
F3, CD142, TF, TFA, coagulation factor III, tissue factor
UniProt
P20352
Reactivity
Mouse
Target
TF
Field of Research
Metabolism Research, Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
3.75 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TF. You can calculate the concentration of Mouse TF in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
6.25-400pg/mL

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