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Human KRT1 (Keratin 1) Microsample ELISA Kit

CAT: 0013-GTR18872785-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872785-01Size:48 Tests
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Description
Human KRT1 (Keratin 1) Microsample ELISA Kit
Product Name Alternative
CK1; EHK1; K1; KRT1A; KRT1-A; Cytokeratin 1; Epidermolytic Hyperkeratosis; Keratin, type II cytoskeletal 1; 67 kDa cytokeratin; Type-II keratin Kb1
UniProt
P04264
Reactivity
Human
Field of Research
Cancer Biology, Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.06 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P04264

Keratin, type II cytoskeletal 1

K2C1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04264
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KRT1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2RA01, P85925, P86104, Q14720, Q6GSJ0, Q9H298
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Cell membraneCytoplasmIntermediate filamentKeratinMembrane

PTM

CitrullinationMethylationPhosphoprotein

Domain

Coiled coil

Disease

Disease variantIchthyosisPalmoplantar keratoderma

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