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Human KRT2 (Keratin 2) Microsample ELISA Kit

CAT: 0013-GTR18872182-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872182-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human KRT2 (Keratin 2) Microsample ELISA Kit
Product Name Alternative
KRT2A; CK2; KRTE; Cytokeratin 2; Keratin 2A; Keratin 2E; Epidermal Ichthyosis Bullosa Of Siemens; Keratin, type II cytoskeletal 2 epidermal; Epithelial keratin-2e
UniProt
P35908
Reactivity
Human
Field of Research
Cancer Biology, Infectious Disease & Virology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity
0.056 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

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