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Human TRA2b (Transformer 2 Beta) Microsample ELISA Kit

CAT: 0013-GTR18871872-02Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18871872-02Size:96 Tests
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Description
Human TRA2b (Transformer 2 Beta) Microsample ELISA Kit
Product Name Alternative
SRFS10; Htra2-beta; TRA2-BETA; TRA2B; Splicing Factor, Arginine/Serine-Rich 10; Transformer-2 protein homolog B
UniProt
P62995
Reactivity
Human
Field of Research
Signal Transduction
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.055 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRA2b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRA2b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRA2b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRA2b in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P62995

Transformer-2 protein homolog beta

TRA2B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P62995
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TRA2B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
TRA-2 beta, TRA2-beta, hTRA2-beta
EC number
—
Processing
—
Secondary accessions
B4DVK2, D3DNU3, O15449, Q15815, Q64283
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondMethylationPhosphoproteinUbl conjugation

Molecular function

ActivatorRepressorRNA-binding

Coding sequence diversity

Alternative splicing

Disease

EpilepsyIntellectual disability

Biological process

mRNA processingmRNA splicing

Cellular component

Nucleus

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