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Human DEFb2 (Defensin Beta 2) Microsample ELISA Kit

CAT: 0013-GTR18873093-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873093-01Size:48 Tests
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Description
Human DEFb2 (Defensin Beta 2) Microsample ELISA Kit
Product Name Alternative
B-DF2; DEFB2; BD2; DEF-B2; HBD2; DEFB102; HBD-2; SAP1; DEFB4A; DEFB4; Skin-Antimicrobial Peptide 1
UniProt
O15263
Reactivity
Human
Field of Research
Infectious Disease & Virology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
13 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DEFb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DEFb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DEFb2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
31.25-2000 pg/mL

UniProtKB · O15263

Defensin beta 4A

DFB4A_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O15263
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
DEFB4A, DEFB4B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q52LC0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

AntibioticAntimicrobialDefensin

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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