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Mouse LIPH (Lipase H) Microsample ELISA Kit

CAT: 0013-GTR18871417-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18871417-01Size:48 Tests
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Description
Mouse LIPH (Lipase H) Microsample ELISA Kit
Product Name Alternative
LPDLR; MPAPLA1; PLA1B; mPA-PLA1; LPD lipase-related protein; Phospholipase A1 member B; Membrane-associated phosphatidic acid-selective phospholipase A1-alpha
UniProt
Q8CIV3
Reactivity
Mouse
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Sensitivity
0.067 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LIPH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LIPH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LIPH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LIPH in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · Q8CIV3

Lipase member H

LIPH_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q8CIV3
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Liph
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.1.1.-
Processing
Precursor
Secondary accessions
Q3TRT3, Q3TTZ0, Q3UWA2, Q8BXB5, Q8CI45
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecreted

PTM

Disulfide bondGlycoprotein

Molecular function

Hydrolase

Biological process

Lipid degradationLipid metabolism

Technical term

Reference proteome

Domain

Signal

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