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Human Platelet Factor 4 (PF4) ELISA Kit

CAT: 0013-GTR18864267-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18864267-01Size:48 Tests
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Description
Human Platelet Factor 4 (PF4) ELISA Kit
Product Name Alternative
CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact
UniProt
P02776
Reactivity
Human
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, platelet-poor plasma and cell culture supernates
Sensitivity
6.5 ng/mL
Notes
For research use only.
Applications Notes
Standard: 1000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PF4 in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
15.63-1000 ng/mL

UniProtKB · P02776

Platelet factor 4

PLF4_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P02776
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PF4
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PF-4
EC number
—
Processing
Precursor
Secondary accessions
Q53X61, Q9UC64, Q9UC65
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Chemotaxis

Molecular function

CytokineHeparin-binding

PTM

Disulfide bondPhosphoprotein

Cellular component

Secreted

Domain

Signal

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