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Mouse Platelet Factor 4 (PF4) ELISA Kit

CAT: 0013-GTR18854798-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854798-01Size:48 Tests
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Description
Mouse Platelet Factor 4 (PF4) ELISA Kit
Product Name Alternative
CXCL4
UniProt
Q9Z126
Reactivity
Mouse
Target
PF4
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
18.75 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PF4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PF4. You can calculate the concentration of Mouse PF4 in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
31.25-2000pg/mL

UniProtKB · Q9Z126

Platelet factor 4

PLF4_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9Z126
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Pf4
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
PF-4
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Chemotaxis

Molecular function

CytokineHeparin-binding

PTM

Disulfide bondGlycoproteinPhosphoprotein

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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