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Human Endoglin (ENG) ELISA Kit

CAT: 0013-GTR18863476-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863476-01Size:48 Tests
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Description
Human Endoglin (ENG) ELISA Kit
Product Name Alternative
CD105; END; HHT1; ORW; ORW1; Osler-Rendu-Weber Syndrome 1
UniProt
P17813
Reactivity
Human
Field of Research
Cardiovascular Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.39 ng/mL
Notes
For research use only.
Applications Notes
Standard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ENG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ENG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ENG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ENG in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
7.81-500 ng/mL

UniProtKB · P17813

Endoglin

EGLN_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P17813
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ENG
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q14248, Q14926, Q5T9C0
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

AngiogenesisCell adhesion

Cellular component

Cell membraneMembrane

Disease

Disease variant

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

SignalTransmembraneTransmembrane helix

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