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Mouse Endoglin (ENG) ELISA Kit

CAT: 0013-GTR18864288-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18864288-01Size:48 Tests
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Description
Mouse Endoglin (ENG) ELISA Kit
Product Name Alternative
CD105; END; HHT1; ORW; ORW1; Osler-Rendu-Weber Syndrome 1
UniProt
Q63961
Reactivity
Mouse
Field of Research
Cardiovascular Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
0.352 ng/mL
Notes
For research use only.
Applications Notes
Standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ENG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ENG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ENG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ENG in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
0.79-50 ng/mL

UniProtKB · Q63961

Endoglin

EGLN_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q63961
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Eng
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q61520, Q8K100
Protein keywords

Biological process

AngiogenesisCell adhesion

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinPhosphoprotein

Technical term

Reference proteome

Domain

SignalTransmembraneTransmembrane helix

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