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Human Complement Factor H (CFH) ELISA Kit

CAT: 0013-GTR18863450-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863450-01Size:48 Tests
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Description
Human Complement Factor H (CFH) ELISA Kit
Product Name Alternative
CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein
UniProt
P08603
Reactivity
Human
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
0.048 μg/mL
Notes
For research use only.
Applications Notes
Standard: 10 μg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFH in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
0.16-10 μg/mL

UniProtKB · P08603

Complement factor H

CFAH_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08603
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CFH
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A5PL14, P78435, Q14570, Q2TAZ5, Q38G77, Q5TFM3, Q8N708, Q9NU86
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Disease

Age-related macular degenerationDisease variantHemolytic uremic syndrome

Coding sequence diversity

Alternative splicing

Biological process

Complement alternate pathwayHost-virus interactionImmunityInnate immunity

PTM

Disulfide bondGlycoproteinSulfation

Domain

RepeatSignalSushi

Cellular component

Secreted

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