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Cattle Osteocalcin (OC) ELISA Kit

CAT: 0013-GTR18863388-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863388-01Size:48 Tests
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Description
Cattle Osteocalcin (OC) ELISA Kit
Product Name Alternative
BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
UniProt
P02820
Reactivity
Bovine
Field of Research
Metabolism Research, Musculoskeletal & Connective Tissue Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
0.67 ng/mL
Notes
For research use only.
Applications Notes
Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle OC in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
1.57-100 ng/mL

UniProtKB · P02820

Osteocalcin

OSTCN_BOVIN · Bos taurus

View on UniProt ↗
Primary accession
P02820
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BGLAP
Protein existence
1: Evidence at protein level
Organism
Bos taurus (Bovine)
Taxonomy ID
9913
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A7YEW1
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Biological process

Biomineralization

Ligand

CalciumMetal-binding

PTM

Cleavage on pair of basic residuesDisulfide bondGamma-carboxyglutamic acidHydroxylation

Molecular function

Hormone

Cellular component

Secreted

Domain

Signal

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