Rat Osteocalcin (OC) ELISA Kit

CAT: 0013-GTR18809435-01Size: 48 TestsDry Ice: NoHazardous: No

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CAT#:0013-GTR18809435-01Size:48 Tests

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteocalcin (OC) .

Product Name Alternative

BGLAP ELISA Kit, OT ELISA Kit, BGP ELISA Kit, Bone Gla Protein ELISA Kit, Bone Gamma-Carboxyglutamate Protein ELISA Kit, Gamma-carboxyglutamic acid-containing protein ELISA Kit

UniProt

P04640

Reactivity

Rat

Field of Research

Metabolism Research, Musculoskeletal & Connective Tissue Research

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Sensitivity

0.29 ng/mL

Notes

For research use only.

Applications Notes

Standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OC in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.78-50 ng/mL

UniProtKB · P04640

Osteocalcin

OSTCN_RAT · Rattus norvegicus

View on UniProt ↗

Primary accession: P04640
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Bglap
Protein existence: 1: Evidence at protein level
Organism: Rattus norvegicus (Rat)
Taxonomy ID: 10116
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: —

Protein keywords

Biological process

Biomineralization

Ligand

CalciumMetal-binding

PTM

Cleavage on pair of basic residuesDisulfide bondGamma-carboxyglutamic acidHydroxylation

Molecular function

Hormone

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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Alternative Products

Catalog	Name
KLR4204	GENLISA™ Osteocalcin (OC) ELISA
TRV-054768-01	ELISA Kit for Osteocalcin (OC)
KT-51440	Rat Osteocalcin (OC) ELISA
KT-24391	Mouse Osteocalcin (OC) ELISA
RDR-OC-c-01	Canine Osteocalcin (OC) ELISA Kit
RDR-OC-Hu-01	Human Osteocalcin (OC) ELISA Kit
MBS8806604-01	Dog OC (Osteocalcin) ELISA Kit
MBS8801423-01	Mouse OC (Osteocalcin) ELISA Kit
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