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Dog Osteocalcin (OC) ELISA Kit

CAT: 0013-GTR18813199-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18813199-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteocalcin (OC) .
Product Name Alternative
BGLAP ELISA Kit, OT ELISA Kit, BGP ELISA Kit, Bone Gla Protein ELISA Kit, Bone Gamma-Carboxyglutamate Protein ELISA Kit, Gamma-carboxyglutamic acid-containing protein ELISA Kit
UniProt
P81455
Reactivity
Canine
Field of Research
Metabolism Research, Musculoskeletal & Connective Tissue Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
0.252 ng/mL
Notes
For research use only.
Applications Notes
Standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog OC in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.63-40 ng/mL

UniProtKB · P81455

Osteocalcin

OSTCN_CANLF · Canis lupus familiaris

View on UniProt ↗
Primary accession
P81455
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BGLAP
Protein existence
1: Evidence at protein level
Organism
Canis lupus familiaris (Dog)
Taxonomy ID
9615
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Biological process

Biomineralization

Ligand

CalciumMetal-binding

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGamma-carboxyglutamic acid

Molecular function

Hormone

Cellular component

Secreted

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