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Mouse E-Cadherin (E-Cad) ELISA Kit

CAT: 0013-GTR18863316-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863316-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse E-Cadherin (E-Cad) ELISA Kit
Product Name Alternative
CDH1; Arc-1; CD324; CDHE; LCAM; UVO; CAM 120/80; Epithelial Cadherin; Uvomorulin
UniProt
P09803
Reactivity
Mouse
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.132 ng/mL
Notes
For research use only.
Applications Notes
Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
0.32-20 ng/mL

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