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Rat E-Cadherin (E-Cad) ELISA Kit

CAT: 0013-GTR18863337-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863337-01Size:48 Tests
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Description
Rat E-Cadherin (E-Cad) ELISA Kit
Product Name Alternative
CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial
UniProt
Q9R0T4
Reactivity
Rat
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, urine, tissue homogenates and other biological fluids
Sensitivity
0.13 ng/mL
Notes
For research use only.
Applications Notes
Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
0.32-20 ng/mL

UniProtKB · Q9R0T4

Cadherin-1

CADH1_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q9R0T4
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Cdh1
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
O35794, Q9JIV9
Protein keywords

Ligand

CalciumMetal-binding

Biological process

Cell adhesion

Cellular component

Cell junctionCell membraneCytoplasmEndosomeGolgi apparatusMembrane

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinPhosphoproteinUbl conjugation

Technical term

Reference proteome

Domain

RepeatSignalTransmembraneTransmembrane helix

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