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Dog Apolipoprotein E (APOE) ELISA Kit

CAT: 0013-GTR18863360-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863360-01Size:48 Tests
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Description
Dog Apolipoprotein E (APOE) ELISA Kit
Product Name Alternative
Apo-E; AD2; Apoprotein; Alzheimer Disease 2 (E4-Associated, Late Onset
UniProt
P18649
Reactivity
Canine
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids.
Sensitivity
46.7 ng/mL
Notes
For research use only.
Applications Notes
Standard: 5000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog APOE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog APOE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog APOE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog APOE in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
78.13-5000 ng/mL

UniProtKB · P18649

Apolipoprotein E

APOE_CANLF · Canis lupus familiaris

View on UniProt ↗
Primary accession
P18649
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
APOE
Protein existence
1: Evidence at protein level
Organism
Canis lupus familiaris (Dog)
Taxonomy ID
9615
Alternative names
Apo-E
EC number
—
Processing
Precursor
Secondary accessions
F1PJ74
Protein keywords

Cellular component

ChylomicronEndosomeExtracellular matrixHDLSecretedVLDL

Technical term

Direct protein sequencingReference proteome

PTM

GlycoproteinOxidationPhosphoprotein

Molecular function

Heparin-binding

Biological process

Lipid transportTransport

Ligand

Lipid-binding

Domain

RepeatSignal

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