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Rat Apolipoprotein E (ApoE) ELISA Kit

CAT: 0013-GTR18854049-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854049-01Size:48 Tests
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Description
Rat Apolipoprotein E (ApoE) ELISA Kit
Product Name Alternative
Apo-E, MGC1571, apolipoprotein E3
UniProt
P02650
Reactivity
Rat
Target
APOE
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
1.88 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ApoE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ApoE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ApoE. You can calculate the concentration of Rat ApoE in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
3.13-200ng/mL

UniProtKB · P02650

Apolipoprotein E

APOE_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P02650
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Apoe
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
Apo-E
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Cellular component

ChylomicronEndosomeExtracellular matrixHDLSecretedVLDL

Technical term

Direct protein sequencingReference proteome

PTM

GlycoproteinOxidationPhosphoprotein

Molecular function

Heparin-binding

Biological process

Lipid transportTransport

Ligand

Lipid-binding

Domain

RepeatSignal

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