Products for Research Use Only

Mouse B-cell Activating Factor (BAFF/CD257) ELISA Kit

CAT: 0013-GTR18859232-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18859232-01Size:48 Tests
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Description
Mouse B-cell Activating Factor (BAFF/CD257) ELISA Kit
Product Name Alternative
TNFSF13B, BLYS, TALL1, THANK, TNFSF2, ZTNF4
UniProt
Q9WU72
Reactivity
Mouse
Target
BAFF/CD257
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
37.5 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BAFF/CD257. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BAFF/CD257 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BAFF/CD257, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse BAFF/CD257. You can calculate the concentration of Mouse BAFF/CD257 in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
62.5-4000pg/mL

UniProtKB · Q9WU72

Tumor necrosis factor ligand superfamily member 13B

TN13B_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9WU72
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Tnfsf13b
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q7TQ58
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecreted

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Molecular function

Cytokine

Technical term

Reference proteome

Domain

Signal-anchorTransmembraneTransmembrane helix