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Human B-Cell Activating Factor (BAFF/CD257) ELISA Kit

CAT: 0013-GTR18808431-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18808431-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell Activating Factor (BAFF) .
Product Name Alternative
CD257 ELISA Kit, TNFSF13B ELISA Kit, BLYS ELISA Kit, TALL1 ELISA Kit, THANK ELISA Kit, TNFSF20 ELISA Kit, ZTNF4 ELISA Kit, B lymphocyte stimulator ELISA Kit, Tumor Necrosis Factor Ligand Superfamily Member 13B ELISA Kit, Dendritic cell-derived TNF-like ELISA Kit
UniProt
Q9Y275
Reactivity
Human
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
28 pg/mL
Notes
For research use only.
Applications Notes
Standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BAFF/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BAFF/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BAFF/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BAFF/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
78.13-5000 pg/mL

UniProtKB · Q9Y275

Tumor necrosis factor ligand superfamily member 13B

TN13B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9Y275
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TNFSF13B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
E0ADT7, Q6FHD6, Q7Z5J2
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecreted

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Molecular function

Cytokine

Biological process

Immunity

Domain

Signal-anchorTransmembraneTransmembrane helix

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