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Human B-Cell Activating Factor (BAFF/CD257) ELISA Kit

CAT: 0013-GTR18854619-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854619-01Size:48 Tests
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Description
Human B-Cell Activating Factor (BAFF/CD257) ELISA Kit
Product Name Alternative
TNFSF13B, BLYS, TALL1, THANK, TNFSF2, ZTNF4
UniProt
Q9Y275
Reactivity
Human
Target
BAFF/CD257
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
25.2 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BAFF/CD257. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAFF/CD257 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAFF/CD257, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BAFF/CD257. You can calculate the concentration of Human BAFF/CD257 in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
62.5-4000pg/mL

UniProtKB · Q9Y275

Tumor necrosis factor ligand superfamily member 13B

TN13B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9Y275
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TNFSF13B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
E0ADT7, Q6FHD6, Q7Z5J2
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecreted

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Molecular function

Cytokine

Biological process

Immunity

Domain

Signal-anchorTransmembraneTransmembrane helix

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