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Mouse Haptoglobin (HP) ELISA Kit

CAT: 0013-GTR18854899-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854899-01Size:48 Tests
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Description
Mouse Haptoglobin (HP) ELISA Kit
Product Name Alternative
Hpt, BP, Hp2-Alpha, HPA1S
UniProt
Q61646
Reactivity
Mouse
Target
HP
Field of Research
Metabolism Research, Neuroscience
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
46.88 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HP. You can calculate the concentration of Mouse HP in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
78.13-5000ng/mL

UniProtKB · Q61646

Haptoglobin

HPT_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q61646
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Hp
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Acute phaseImmunity

Molecular function

AntibioticAntimicrobialAntioxidantSerine protease homolog

PTM

Disulfide bondGlycoprotein

Ligand

Hemoglobin-binding

Technical term

Reference proteome

Cellular component

Secreted

Domain

SignalSushi

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