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Human Haptoglobin (HP) ELISA Kit

CAT: 0013-GTR18808002-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18808002-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Haptoglobin (Hpt) .
Product Name Alternative
HP ELISA Kit, Hp2-Alpha ELISA Kit, Alpha-2-Macroglobulin ELISA Kit, Zonulin ELISA Kit
UniProt
P00738
Reactivity
Human
Field of Research
Hematology, Infectious Disease & Virology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
2.92 ng/mL
Notes
For research use only.
Applications Notes
Standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HP in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
6.25-400 ng/mL

UniProtKB · P00738

Haptoglobin

HPT_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P00738
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HP
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B0AZL5, P00737, Q0VAC4, Q0VAC5, Q2PP15, Q3B7J0, Q6LBY9, Q9UC67
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Acute phaseImmunity

Coding sequence diversity

Alternative splicing

Molecular function

AntibioticAntimicrobialAntioxidantSerine protease homolog

Disease

Disease variant

PTM

Disulfide bondGlycoprotein

Ligand

Hemoglobin-binding

Domain

RepeatSignalSushi

Cellular component

Secreted

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