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Rat Prolactin (PRL) Microsample Fast ELISA Kit

CAT: 0013-GTR18853742-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18853742-01Size:48 Tests
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Description
Rat Prolactin (PRL) Microsample Fast ELISA Kit
Product Name Alternative
Lactotrope, LTH, Luteotropic Hormone
UniProt
P01237
Reactivity
Rat
Target
PRL
Field of Research
Cancer Biology, Signal Transduction
Assay Type
Sandwich
Assay Performance Time
3H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples; Sample volume:25μL
Sensitivity
1.88 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PRL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PRL. You can calculate the concentration of Rat PRL in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
3.13-200ng/mL

UniProtKB · P01237

Prolactin

PRL_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P01237
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Prl
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
PRL
EC number
—
Processing
Precursor
Secondary accessions
P70520
Protein keywords

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondPhosphoprotein

Molecular function

Hormone

Biological process

Lactation

Cellular component

Secreted

Domain

Signal

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