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Rat Prolactin (PRL) ELISA Kit

CAT: 0013-GTR18813218-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18813218-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolactin (PRL) .
Product Name Alternative
LTH ELISA Kit, Luteotropic Hormone ELISA Kit
UniProt
P01237
Reactivity
Rat
Field of Research
Metabolism Research, Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.122 ng/mL
Notes
For research use only.
Applications Notes
Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PRL in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.32-20 ng/mL

UniProtKB · P01237

Prolactin

PRL_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P01237
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Prl
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
PRL
EC number
—
Processing
Precursor
Secondary accessions
P70520
Protein keywords

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondPhosphoprotein

Molecular function

Hormone

Biological process

Lactation

Cellular component

Secreted

Domain

Signal

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