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HighFidelity Cy5 PCR Labeling Kit

CAT: 0013-GTR18800340Size: 50 rxn x 20 ulDry Ice: NoHazardous: No
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CAT#:0013-GTR18800340Size:50 rxn x 20 ul
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HighFidelity Cy5 PCR Labeling Kit is designed to produce randomly Cy5-modified DNA probes by PCR. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4 kbp is feasible. dUTP-Cy5 is efficiently incorporated into DNA as substitute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taqpolymerase and a proofreading enzyme. 50% dUTP-Cy5 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of dUTP-Cy5/dTTP ratio however, can easily be achieved with the single nucleotide format. The kit contains sufficient reagents for 10 labeling reactions (S-Pack) or 50 labeling reactions (L-Pack) of 20 μl each (50% dUTP-Cy5 substitution, 100 μM dATP/dGTP/dCTP, 50 μM dTTP, 50 μM dUTP-Cy5) .
Product Name Alternative
Preparation of Cy5-labeled DNA probes by PCR
Field of Research
Molecular Biology
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Spectroscopic Propertie: λexc 649 nm, λem 670 nm, ε 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) .

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