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HighFidelity LNA PCR Labeling Kit

CAT: 0013-GTR18800291Size: 40 rxn x 20 ulDry Ice: NoHazardous: No
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CAT#:0013-GTR18800291Size:40 rxn x 20 ul
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HighFidelity LNA PCR Labeling Kit is designed to produce randomly Locked nucleic acid (LNA) -modified DNA probes by PCR. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. The ribose ring of locked nucleic acids is "locked" in the ideal conformation for Watson-Crick binding. LNA-modified DNA probes therefore possess an unprecedented thermal stability upon hy-bridization resulting in an increased sensitivity. LNA-UTP, LNA-CTP, LNA-ATP and LNA-GTP are efficiently incor-porated into DNA as substitute for their natural counterpart (dTTP, dCTP, dATP or dGTP, respectively) using an optimized reaction buffer and a High Fidelity Polymerase. 50% LNA-NTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of LNA-NTP/dNTP ra-tio however, can easily be achieved with the single nucleotide format. The kit contains sufficient reagents for 10 labeling reactions each LNA-NTP (20 μl each (50% LNA-NTP substitution) .
Product Name Alternative
Preparation of LNA-labeled DNA probes by PCR
Field of Research
Molecular Biology
Storage Conditions
Store at -20 °C
Notes
For research use only.

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