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5 (6) -FITC

CAT: 0804-HY-15941-01Size: 100 mgDry Ice: NoHazardous: No
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CAT#:0804-HY-15941-01Size:100 mg
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Description
5 (6) -FITC (Fluorescein 5 (6) -isothiocyanate) is an amine-reactive derivative of a fluorescent dye, characterized by high absorbance and excellent fluorescence quantum yield. The isothiocyanate group of FITC can react with various functional groups on proteins, including amines, thiols, imidazoles, tyrosines and carbonyls, enabling the labeling of proteins such as antibodies and lectins. 5 (6) -FITC has a wide range of applications, including flow cytometry, immunofluorescence, protease assays and conjugation. The maximum excitation/emission wavelengths are 492/518 nm[1][2][3][4].
CAS Number
27072-45-3
Product Name Alternative
Fluorescein 5 (6) -isothiocyanate; Fluorescein isothiocyanate 5- and 6- isomers
UNSPSC
12171500
Hazard Statement
H302+H332, H315, H317, H319, H334, H335
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/5_6_-FITC.html
Purity
97.59
Solubility
DMSO : ≥ 156 mg/mL
Smiles
OC1=CC=C(C2(O3)C(C=C(N=C=S)C=C4)=C4C3=O)C(OC5=C2C=CC(O)=C5)=C1.OC6=CC=C(C7(O8)C(C=CC(N=C=S)=C9)=C9C8=O)C(OC%10=C7C=CC(O)=C%10)=C6
Molecular Formula
C21H11NO5S
Molecular Weight
389.38
Precautions
H302+H332, H315, H317, H319, H334, H335
References & Citations
[1]J S Miller , ET AL. Flow cytometric identification of microorganisms by dual staining with FITC and PI. Cytometry. 1990;11 (6) :667-75.|[2]S S Twining, et al. Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes.Anal Biochem. 1984 Nov 15;143 (1) :30-4.|[3]K Sugiura, et al. A simple quantitative immunofluorescence assay for FITC-labeled antibody on platelets. J Immunol Methods. 1981;40 (2) :165-70.|[4]Natalia Oddone, et al. Cell uptake mechanisms of PAMAM G4-FITC dendrimer in human myometrial cells. J Nanopart Res. 2013 15:1776.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C (Powder, protect from light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

Chemical Information

3',6'-Dihydroxy-5(or 6)-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one

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CAS Number27072-45-3
PubChem CID56973708
IUPAC Name3',6'-dihydroxy-5-isothiocyanatospiro[2-benzofuran-3,9'-xanthene]-1-one;3',6'-dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9'-xanthene]-1-one
Molecular FormulaC42H22N2O10S2
Molecular Weight778.8
Topological Polar Surface Area241
Hydrogen Bond Donor Count4
Hydrogen Bond Acceptor Count14
Rotatable Bond Count2
Heavy Atom Count56
Formal Charge0
Complexity1340
SMILES
C1=CC2=C(C=C1N=C=S)C3(C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O)OC2=O.C1=CC2=C(C=C1N=C=S)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
InChI
InChI=1S/2C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21;23-12-2-5-15-18(8-12)26-19-9-13(24)3-6-16(19)21(15)17-7-11(22-10-28)1-4-14(17)20(25)27-21/h2*1-9,23-24H
InChIKey
AEWCEHLYVLRLBF-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of 3',6'-Dihydroxy-5(or 6)-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
CAS: 27072-45-3
CID: 56973708
Formula: C42H22N2O10S2
MW: 778.8
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight778.8
Hydrogen Bond Donor Count4
Hydrogen Bond Acceptor Count14
Rotatable Bond Count2
Exact Mass778.07158725
Monoisotopic Mass778.07158725
Topological Polar Surface Area241 Ų
Heavy Atom Count56
Formal Charge0
Complexity1340
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count2
Compound Is CanonicalizedYes

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